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bio-restriction-golden-gate-assembly

85 / 100 · Low risk

Fine to try.

By lilinji/GeneTind-Life-Skills (GitHub) · design · 17 stars · scanned at commit 908229c

Design and validate Type IIS scarless DNA assembly (Golden Gate, MoClo) using Biopython Bio.Restriction. Screens parts for internal BsaI/BsmBI/BbsI/SapI sites (domestication), previews the fusion overhangs a digest exposes, and validates a fusion-overhang set for distinctness and fidelity. Use when designing a Golden Gate or MoClo assembly, domesticating a part by removing internal Type IIS sites, or choosing and checking fusion overhangs for one-pot assembly.

Works with any agent that supports SKILL.md skills.

What this skill can do

Install

Paste this into your terminal. It downloads exactly the version we scanned, not whatever is newest.

if [ -e ~/.agents/skills/'bio-restriction-golden-gate-assembly' ] || [ -L ~/.agents/skills/'bio-restriction-golden-gate-assembly' ]; then echo 'A skill folder named bio-restriction-golden-gate-assembly already exists — not replacing it' >&2; false; else d=$(mktemp -d) && git clone --quiet --filter=blob:none --no-checkout 'https://github.com/lilinji/GeneTind-Life-Skills' "$d" && git -C "$d" checkout --quiet '908229c955510a1841b216e8bf58f6b22818d4dc' -- 'skills/bio-restriction-golden-gate-assembly' && mkdir -p ~/.agents/skills && cp -R "$d"/'skills/bio-restriction-golden-gate-assembly' ~/.agents/skills/'bio-restriction-golden-gate-assembly' && rm -rf "$d"; fi

Codex reads ~/.agents/skills directly. For Claude Code, Cursor, Copilot, Gemini CLI, Antigravity or OpenCode, also link it. For Claude Code: ln -s ~/.agents/skills/'bio-restriction-golden-gate-assembly' ~/.claude/skills/'bio-restriction-golden-gate-assembly' (other agents: ~/.cursor/skills, ~/.copilot/skills, ~/.gemini/skills, ~/.gemini/config/skills, ~/.config/opencode/skills). Or install through the finder skill, which does both.

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