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bio-primer-design-qpcr-primers

85 / 100 · Low risk

Fine to try.

By lilinji/GeneTind-Life-Skills (GitHub) · design · 17 stars · scanned at commit 908229c

Co-designs qPCR/RT-qPCR primers and hydrolysis (TaqMan) or molecular-beacon probes with primer3-py (PRIMER_PICK_INTERNAL_OLIGO, PRIMER_INTERNAL_* tags), for assays whose deliverable is a quantitative measurement device. Covers why amplification efficiency (90-110%, slope -3.6 to -3.1) and single-product specificity make the 2^-ddCq / Pfaffl math valid, why the short amplicon (70-150 bp), tight Tm, and zero-dimer requirement exist, the coupled probe rules (probe Tm 8-10 C above primers so it is …

Works with any agent that supports SKILL.md skills.

What this skill can do

Install

Paste this into your terminal. It downloads exactly the version we scanned, not whatever is newest.

if [ -e ~/.agents/skills/'bio-primer-design-qpcr-primers' ] || [ -L ~/.agents/skills/'bio-primer-design-qpcr-primers' ]; then echo 'A skill folder named bio-primer-design-qpcr-primers already exists — not replacing it' >&2; false; else d=$(mktemp -d) && git clone --quiet --filter=blob:none --no-checkout 'https://github.com/lilinji/GeneTind-Life-Skills' "$d" && git -C "$d" checkout --quiet '908229c955510a1841b216e8bf58f6b22818d4dc' -- 'skills/bio-primer-design-qpcr-primers' && mkdir -p ~/.agents/skills && cp -R "$d"/'skills/bio-primer-design-qpcr-primers' ~/.agents/skills/'bio-primer-design-qpcr-primers' && rm -rf "$d"; fi

Codex reads ~/.agents/skills directly. For Claude Code, Cursor, Copilot, Gemini CLI, Antigravity or OpenCode, also link it. For Claude Code: ln -s ~/.agents/skills/'bio-primer-design-qpcr-primers' ~/.claude/skills/'bio-primer-design-qpcr-primers' (other agents: ~/.cursor/skills, ~/.copilot/skills, ~/.gemini/skills, ~/.gemini/config/skills, ~/.config/opencode/skills). Or install through the finder skill, which does both.

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