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bio-primer-design-primer-specificity

82 / 100 · Low risk

Fine to try.

By lilinji/GeneTind-Life-Skills (GitHub) · design · 17 stars · scanned at commit 908229c

Checks whether a PCR primer PAIR amplifies only the intended target genome-wide, using pair-aware in-silico PCR (MFEprimer-3.0, UCSC isPcr, NCBI Primer-BLAST) plus a primer3-py 3'-end-stability prefilter, against the correct database. Covers why plain BLAST is the wrong tool (it scores per-primer similarity, blind to 3'-terminal anchoring and to whether the two primers form a convergent amplicon in range), why a single 3'-terminal mismatch suppresses amplification while internal mismatches are …

Works with any agent that supports SKILL.md skills.

What this skill can do

Install

Paste this into your terminal. It downloads exactly the version we scanned, not whatever is newest.

if [ -e ~/.agents/skills/'bio-primer-design-primer-specificity' ] || [ -L ~/.agents/skills/'bio-primer-design-primer-specificity' ]; then echo 'A skill folder named bio-primer-design-primer-specificity already exists — not replacing it' >&2; false; else d=$(mktemp -d) && git clone --quiet --filter=blob:none --no-checkout 'https://github.com/lilinji/GeneTind-Life-Skills' "$d" && git -C "$d" checkout --quiet '908229c955510a1841b216e8bf58f6b22818d4dc' -- 'skills/bio-primer-design-primer-specificity' && mkdir -p ~/.agents/skills && cp -R "$d"/'skills/bio-primer-design-primer-specificity' ~/.agents/skills/'bio-primer-design-primer-specificity' && rm -rf "$d"; fi

Codex reads ~/.agents/skills directly. For Claude Code, Cursor, Copilot, Gemini CLI, Antigravity or OpenCode, also link it. For Claude Code: ln -s ~/.agents/skills/'bio-primer-design-primer-specificity' ~/.claude/skills/'bio-primer-design-primer-specificity' (other agents: ~/.cursor/skills, ~/.copilot/skills, ~/.gemini/skills, ~/.gemini/config/skills, ~/.config/opencode/skills). Or install through the finder skill, which does both.

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