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bio-primer-design-primer-basics

85 / 100 · Low risk

Fine to try.

By lilinji/GeneTind-Life-Skills (GitHub) · design · 17 stars · scanned at commit 908229c

Designs and ranks PCR primer pairs for a target template with primer3-py (design_primers), returning pairs with nearest-neighbor Tm, GC, product size, and complementarity scores. Covers why primer3 is a LOCAL weighted-penalty minimizer over the single template supplied (so PRIMER_PAIR_0 is the lowest-penalty pair under the given bounds, never a genome-specificity guarantee), why Tm is a salt/concentration-dependent SantaLucia prediction not a fixed property, why the two primers must be Tm-match…

Works with any agent that supports SKILL.md skills.

What this skill can do

Install

Paste this into your terminal. It downloads exactly the version we scanned, not whatever is newest.

if [ -e ~/.agents/skills/'bio-primer-design-primer-basics' ] || [ -L ~/.agents/skills/'bio-primer-design-primer-basics' ]; then echo 'A skill folder named bio-primer-design-primer-basics already exists — not replacing it' >&2; false; else d=$(mktemp -d) && git clone --quiet --filter=blob:none --no-checkout 'https://github.com/lilinji/GeneTind-Life-Skills' "$d" && git -C "$d" checkout --quiet '908229c955510a1841b216e8bf58f6b22818d4dc' -- 'skills/bio-primer-design-primer-basics' && mkdir -p ~/.agents/skills && cp -R "$d"/'skills/bio-primer-design-primer-basics' ~/.agents/skills/'bio-primer-design-primer-basics' && rm -rf "$d"; fi

Codex reads ~/.agents/skills directly. For Claude Code, Cursor, Copilot, Gemini CLI, Antigravity or OpenCode, also link it. For Claude Code: ln -s ~/.agents/skills/'bio-primer-design-primer-basics' ~/.claude/skills/'bio-primer-design-primer-basics' (other agents: ~/.cursor/skills, ~/.copilot/skills, ~/.gemini/skills, ~/.gemini/config/skills, ~/.config/opencode/skills). Or install through the finder skill, which does both.

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