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bio-alignment-filtering
80 / 100 · Low risk
Fine to try.
By diegosouzapw/awesome-omni-skill (GitHub) · other · 66 stars · scanned at commit a6b3c30
Filter alignments by flags, mapping quality, and regions using samtools view and pysam. Use when extracting specific reads, removing low-quality alignments, or subsetting to target regions.
Works with any agent that supports SKILL.md skills.
What this skill can do
Runs commands on your computer
The author does not mention this.
Found in 21 places.
Show 17 more
- skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 58
samtools view -F 1024 -o nodup.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 63
samtools view -F 2304 -o primary.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 68
samtools view -F 256 -F 2048 -o primary.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 74
samtools view -f 64 -o read1.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 79
samtools view -f 128 -o read2.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 84
samtools view -F 16 -o forward.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 89
samtools view -f 16 -o reverse.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 96
samtools view -q 30 -o highqual.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 101
samtools view -F 4 -q 30 -o filtered.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 118
samtools view -o region.bam input.bam chr1:1000000-2000000 - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 123
samtools view -o regions.bam input.bam chr1:1000-2000 chr2:3000-4000 - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 128
samtools view -L targets.bed -o targets.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 133
samtools view -q 30 -L targets.bed -o filtered.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 141
samtools view -F 3332 -q 30 -o filtered.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 148
samtools view -f 2 -F 3328 -q 20 -o clean.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 156
samtools view -F 1024 -q 30 -o filtered.bam input.bam - skills/fullstack-web/bio-alignment-filtering/SKILL.md, line 164
samtools view -s 0.1 -o subset.bam input.bam
and 1 more places
Sends data to these websites: raw.githubusercontent.com
The author does not mention this.
Found in 3 places.
Changes files outside its own folder
The author does not mention this.
Found in 1 place.
Install
Paste this into your terminal. It downloads exactly the version we scanned, not whatever is newest.
if [ -e ~/.agents/skills/'bio-alignment-filtering' ] || [ -L ~/.agents/skills/'bio-alignment-filtering' ]; then echo 'A skill folder named bio-alignment-filtering already exists — not replacing it' >&2; false; else d=$(mktemp -d) && git clone --quiet --filter=blob:none --no-checkout 'https://github.com/diegosouzapw/awesome-omni-skill' "$d" && git -C "$d" checkout --quiet 'a6b3c3005cedf056a48b084671c6282426d6e9fc' -- 'skills/fullstack-web/bio-alignment-filtering' && mkdir -p ~/.agents/skills && cp -R "$d"/'skills/fullstack-web/bio-alignment-filtering' ~/.agents/skills/'bio-alignment-filtering' && rm -rf "$d"; fi
Codex reads ~/.agents/skills directly. For Claude Code, Cursor, Copilot, Gemini CLI, Antigravity or OpenCode, also link it. For Claude Code: ln -s ~/.agents/skills/'bio-alignment-filtering' ~/.claude/skills/'bio-alignment-filtering' (other agents: ~/.cursor/skills, ~/.copilot/skills, ~/.gemini/skills, ~/.gemini/config/skills, ~/.config/opencode/skills). Or install through the finder skill, which does both.
Badge for your README
The badge for diegosouzapw/awesome-omni-skill shows the score of its lowest-scoring skill.

[](https://zalavijaysinh.github.io/skilltrust/repos/diegosouzapw/awesome-omni-skill/)
Score history
| Date | Version | Score |
|---|
| 2026-11-07 | a6b3c30 | 80 |