← All skills
bio-bedgraph-handling
80 / 100 · Low risk
Fine to try.
By BioTender-max/awesome-bio-agent-skills (GitHub) · design · 219 stars · scanned at commit 8cbdd18
Create, manipulate, and convert bedGraph files for genome browser visualization. Covers bedGraph format, conversion to/from bigWig, normalization, and signal processing. Use when handling coverage and signal tracks from ChIP-seq, ATAC-seq, or RNA-seq.
Works with any agent that supports SKILL.md skills.
What this skill can do
Runs commands on your computer
The author does not mention this.
Found in 28 places.
Show 17 more
- skills/bioskills/bedgraph-handling/SKILL.md, line 64
total_reads=$(samtools view -c -F 260 sample.bam) - skills/bioskills/bedgraph-handling/SKILL.md, line 75
sort -k1,1 -k2,2n sample.bedgraph > sample.sorted.bedgraph - skills/bioskills/bedgraph-handling/SKILL.md, line 84
bedGraphToBigWig sample.sorted.bedgraph chrom.sizes sample.bw - skills/bioskills/bedgraph-handling/SKILL.md, line 92
samtools faidx reference.fa - skills/bioskills/bedgraph-handling/SKILL.md, line 102
bedClip sample.bedgraph chrom.sizes sample.clipped.bedgraph - skills/bioskills/bedgraph-handling/SKILL.md, line 109
bigWigToBedGraph sample.bw sample.bedgraph - skills/bioskills/bedgraph-handling/SKILL.md, line 119
bedtools unionbedg -i sample1.bedgraph sample2.bedgraph sample3.bedgraph \ - skills/bioskills/bedgraph-handling/SKILL.md, line 126
bedtools unionbedg -i sample1.bedgraph sample2.bedgraph sample3.bedgraph | \ - skills/bioskills/bedgraph-handling/SKILL.md, line 136
bedtools map -a regions.bed -b sample.bedgraph -c 4 -o mean > region_means.bed - skills/bioskills/bedgraph-handling/SKILL.md, line 144
bedtools unionbedg -i treatment.bedgraph input.bedgraph | \ - skills/bioskills/bedgraph-handling/SKILL.md, line 152
awk '{print $1,$2,$3,log($4+1)/log(2)}' OFS='\t' sample.bedgraph > sample.log2.bedgraph - skills/bioskills/bedgraph-handling/SKILL.md, line 158
bedtools slop -i sample.bedgraph -g chrom.sizes -b 50 | \ - skills/bioskills/bedgraph-handling/SKILL.md, line 216
bamCoverage -b sample.bam -o sample.bw --normalizeUsing RPKM - skills/bioskills/bedgraph-handling/SKILL.md, line 225
bamCompare -b1 treatment.bam -b2 input.bam -o log2ratio.bw --scaleFactorsMethod readCount - skills/bioskills/bedgraph-handling/SKILL.md, line 232
bigwigCompare -b1 treatment.bw -b2 input.bw -o ratio.bw --ratio log2 - skills/bioskills/bedgraph-handling/SKILL.md, line 241
awk '$4 >= 1.0' sample.bedgraph > high_signal.bedgraph - skills/bioskills/bedgraph-handling/SKILL.md, line 248
bedtools intersect -a sample.bedgraph -b regions.bed > subset.bedgraph
and 8 more places
Downloads and installs other software
The author does not mention this.
Found in 1 place.
Install
Paste this into your terminal. It downloads exactly the version we scanned, not whatever is newest.
if [ -e ~/.agents/skills/'bio-bedgraph-handling' ] || [ -L ~/.agents/skills/'bio-bedgraph-handling' ]; then echo 'A skill folder named bio-bedgraph-handling already exists — not replacing it' >&2; false; else d=$(mktemp -d) && git clone --quiet --filter=blob:none --no-checkout 'https://github.com/BioTender-max/awesome-bio-agent-skills' "$d" && git -C "$d" checkout --quiet '8cbdd18837aa6296c4e77616a03b323bd69b57b1' -- 'skills/bioskills/bedgraph-handling' && mkdir -p ~/.agents/skills && cp -R "$d"/'skills/bioskills/bedgraph-handling' ~/.agents/skills/'bio-bedgraph-handling' && rm -rf "$d"; fi
Codex reads ~/.agents/skills directly. For Claude Code, Cursor, Copilot, Gemini CLI, Antigravity or OpenCode, also link it. For Claude Code: ln -s ~/.agents/skills/'bio-bedgraph-handling' ~/.claude/skills/'bio-bedgraph-handling' (other agents: ~/.cursor/skills, ~/.copilot/skills, ~/.gemini/skills, ~/.gemini/config/skills, ~/.config/opencode/skills). Or install through the finder skill, which does both.
Badge for your README
The badge for BioTender-max/awesome-bio-agent-skills shows the score of its lowest-scoring skill.

[](https://zalavijaysinh.github.io/skilltrust/repos/BioTender-max/awesome-bio-agent-skills/)
Score history
| Date | Version | Score |
|---|
| 2026-11-07 | 8cbdd18 | 80 |